Preparation and evaluation of a water-soluble cholesterol standard.

We synthesized some inorganic and organic salts of cholesteryl hemisuccinate. Aqueous solutions of the morpholine salt, at concentrations equivalent to 400 mg of cholesterol per deciliter, have been evaluated as aqueous cholesterol standards and compared to alcoholic cholesterol solutions in analyses for cholesterol in serum, as performed by two analytical reference procedures. No significant differences were found. The usefulness of solutions of this salt as additives for serum controls or recovery studies has been also demonstrated.